ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) : ASCP-MLT

  • Exam Code: ASCP
  • Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Updated: Aug 10, 2026
  • Q & A: 562 Questions and Answers

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About ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) : ASCP Exam

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ASCP ASCP-MLT Exam Syllabus Topics:
SectionWeightObjectives
Topic 1: Urinalysis and Other Body Fluids5–10%- Physical and chemical testing
- Microscopic analysis and disease correlation
Topic 2: Hematology20–25%- Laboratory testing and hemostasis
- Physiology and disease states
Topic 3: Immunology5–10%- Principles of immunology
- Serologic procedures and immune diseases
Topic 4: Blood Banking15–20%- Blood products and components
- Blood group systems and immunology
- Transfusion practice and testing
Topic 5: Microbiology15–20%- Bacteriology and mycology procedures
- Virology and parasitology testing
Topic 6: Laboratory Operations5–10%- Quality assessment and safety
- Instrumentation and laboratory mathematics
Topic 7: Chemistry20–25%- Carbohydrates, lipids, and enzymes
- Electrolytes, acid-base, and toxicology
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Which of the following is NOT part of the magnification system of the microscope?

A) 10 X objective lens
B) 4 X objective lens
C) ocular lenses-arm
D) arm


2. The DAT is most likely to be negative in ABO HDFN. It's possible that the washing done as part of the DAT may break the bonds between anti-A (or anti-B) and the newborn's poorly developed A (or B) antigens.
For which of the following antibodies is the DAT most likely to be negative when testing a newborn for possible HDFN?

A) anti-D
B) anti-c
C) anti-Fya
D) anti-K
E) anti-A


3. Twelve weeks after onset of the disease, patients with uncomplicated acute hepatitis B usually will demonstrate which of the following in their serum?

A) Anti-HIV
B) Anti-HTLV
C) Anti-HBe
D) HBsAg


4. Phase of reactivity is primarily at immediate spin (4+) and reactions get weaker at AHG (w+). There is no specific pattern of reactivity and the auto control is negative which rules out an autoantibody. This is a strong cold antibody which is still slightly present after incubation and washing.
Activation and binding of the antibody takes place at room temperature or colder. Eliminating this phase will prevent the antibody from binding. Cold antibodies usually are more of a nuisance to blood bankers and are not clinically significant.
When performing an antibody screen, both the screen cells are 4+ at immediate spin and W+ at AHG. The antibody panel shows 4+ reactions at immediate spin and W+ reactions at AHG and there is no specific match to the reaction pattern. The auto control is negative. What would be a logical next step?

A) Repeat testing using warmed patient sample and reagents and just do AHG reading
B) Run an enzyme panel
C) Have patient redrawn


5. High triglycerides may be caused by disorders such as type 2 diabetes, hypothyroidism, Cushing's sydnrome, liver disease, uremia, dysglobulinemia, nephrotic syndrome, and alcoholism can cause hypertriglyceridemia.
A 46-year old known alcoholic with liver damage is brought in the ER unconscious. One would expect his lipid values to be affected in what way?

A) Normal
B) Unaffected by the alcoholism
C) Increased
D) Decreased


Solutions:

Question # 1
Answer: D
Question # 2
Answer: E
Question # 3
Answer: C
Question # 4
Answer: A
Question # 5
Answer: C

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